Effects of Pluronic F-127 on loading of fura 2/AM into single smooth muscle cells isolated from guinea pig taenia coli.
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چکیده
منابع مشابه
Outward current in single smooth muscle cells of the guinea pig taenia coli
In single myocytes of the guinea pig taenia coli, dispersed by enzymatic digestion, the late outward current is carried by K+. It has both a Ca2+-activated component and a voltage-dependent component which is resistant to external Co2+. The reversal potential is -84 mV, and the channel(s) for it are highly selective to K+. At 33 degrees C, the activation follows n2 kinetics, with a voltage-depe...
متن کاملInward current in single smooth muscle cells of the guinea pig taenia coli
Using the tight-seal voltage-clamp method, the ionic currents in the enzymatically dispersed single smooth muscle cells of the guinea pig taenia coli have been studied. In a physiological medium containing 3 mM Ca2+, the cells are gently tapering spindles, averaging 201 (length) x 8 microns (largest diameter in center of cell), with a volume of 5 pl. The average cell capacitance is 50 pF, and t...
متن کاملIsolation and contractile properties of single smooth muscle cells from guinea pig taenia caeci.
A method for the isolation of single smooth muscle cells from guinea pig taenia caeci is described. The single cells were prepared by digestion with 0.3% collagenase and 0.6% trypsin inhibitor in Ca2+-free solution. This procedure produced a high yield of intact cells. Most cells obtained by this procedure were relaxed and showed large contractile responses to excitatory stimulus. Maximal respo...
متن کاملDesensitization of smooth muscle cells in the guinea pig taenia coli to prolonged application of carbachol.
depolarized the membrane, reduced the membrane resistance and evoked contracture. These responses did not, however, continue but a desensitization to Carb then developed, i. e. the membrane was gradually repolarized, membrane resistance increased, and the muscle relaxed in spite of the presence of Carb. 2. During the repolarization phase in the presence of Carb (10-5 g/ml), repeated current pul...
متن کاملSodium conductance in calcium channels of single smooth muscle cells of guinea-pig taenia caeci.
During the last few years, the Ca 2+-channels in isolated smooth muscle cells have been intensively studied (Walsh and Singer 1981; Bury et al. 1982; Klock ner and Isenberg 1985; Droogmans and Callewaert 1986). It was shown that under physiological conditions Ca 2+-channels are not measurably permeable for Na-ions (Klockner and Isenberg 1985). On the other hand, inward currents carried by Na +...
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ژورنال
عنوان ژورنال: The Journal of Toxicological Sciences
سال: 1989
ISSN: 0388-1350,1880-3989
DOI: 10.2131/jts.14.153